Description
FEATURES
- The kit is based upon Single Step RT-PCR, where both Reverse Transcription by Reverse Transcriptase and PCR by Taq Polymerase takes place in single tube.
- Monkey Pox virus kit is developed using four colour fluorescence technology.
- A signal from different channel signifies, presence of Orthopox group, Monkeypox & Smallpox virus respectively in the sample.
- Assay validation through the additional human internal control gene.
- Compatible with both dry swabs as well as sample swabs placed in VTM.
- A total turnaround time of 1 hour
TECHNICAL SPECIFICATIONS
- The kit has been validated on QuantStudio-5/3 and Bio-Rad CFX96, 384 and can also be used on applied Biosystems 7300/7500, Roche Diagnostics LightCycler 96/480, Qiagen Rotor-Gene Q, etc. (Instrument should be calibrated, maintained and used as per manufacturer recommendation).
- Sample type: Swab samples collected from surface and/or exudate, roofs from more than one lesion, or lesion crusts.
- Storage: -20±2°C